Translational_Unit

Part:BBa_K5410028:Design

Designed by: Zixuan Li   Group: iGEM24_GeorgiaState-SWJTU   (2024-10-02)


phaBCA for E. coli Poly-3-hydroxybutyrate production


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
    Illegal NotI site found at 886
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1328
    Illegal XhoI site found at 133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
    Illegal NgoMIV site found at 94
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 472
    Illegal NgoMIV site found at 939
    Illegal NgoMIV site found at 1054
    Illegal NgoMIV site found at 1288
    Illegal NgoMIV site found at 1879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

However, the position of the three genes relative to the promoter affects the molecular weight and yield of PHB. The strain harboring the phaBCA expression plasmid achieved the most balanced production result.


Source

Cupriavidus necator N-1

References